Studies of Ribonucleic Acid Chain Initiation by Escherichia coli Ribonucleic Acid Polymerase Bound to T7 Deoxyribonucleic Acid
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چکیده
منابع مشابه
Kinetic analysis of ribonucleic acid chain initiation by Escherichia coli Ribonucleic acid polymerase bound to DNA.
The kinetics of the RNA chain initiation reaction carried out by RNA polymerase bound to the initiator region of a DNA template have been analyzed. Initiation proceeds in a two-substrate reaction in which the initial binary complex (enzyme-DNA) is transformed into a ternary complex (enzyme-DNA-RNA) by formation of a dinucleoside tetraphosphate and release of inorganic pyrophosphate. In this rea...
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RNA polymerase core and holoenzyme isolated from Escherichia coli have been denatured and reconstituted in high yield (70 to 100%). Ultracentrifugation studies and measurement of tryptophan fluorescence indicate that the reconstituted enzyme has a structure similar to that of the native enzyme. The reconstituted holoenzyme transcribes T7 DNA as efficiently as the native holoenzyme. RNA synthesi...
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Subunits of RNA polymerase core enzyme, separated by chromatography on phosphocellulose in 7 M urea, can be reconstituted to form core enzyme with a 40 to 60% yield. Reconstitution of active enzyme from subunits requires their codialysis while subunits reconstituted individually and then mixed do not yield active enzyme. Subunits 0 and (Y cause retention of labeled ‘I7 DNA by nitrocellulose fil...
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The fidelity of in vitro transcription of bacteriophage T3 DNA by T3 RNA polymerase and Escherichia coZi RNA POlymerase have been studied by DNA-RNA hybridizationcompetition studies with regard to (a) asymmetry of transcription and (b) the region of the T3 DNA transcribed by either polymerase. RNA transcribed by T3 RNA polymerase hybridized exclusively with the H strand of T3 DNA-the only stran...
متن کاملDeoxyribonucleic acid-ribonucleic acid hybridization studies on the L-Arabinose operon of Escherichia coli B-r.
An increase in the rate of synthesis of ara-specific messenger ribonucleic acid as measured by deoxyribonucleic acid-ribonucleic acid hybridization has been detected in the induced wild-type (ara(+)) strain of Escherichia coli B/r as compared with the uninduced control, thus providing evidence that regulation of the positively controlled l-arabinose operon is at the level of transcription.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)42629-x